Analyst adds enriched sample to the inoculation chamber and a drop of the 1-2 Test antibody to the void in the peptone-based gel in the motility chamber. The 1-2 Test is incubated. |
Salmonella, if present, begins to move through the selective medium into the motility chamber. At the same time, the 1-2 Test antibody starts diffusing into the motility chamber in a radial pattern |
As the Salmonella encounters the diffusing antibodies they are immobilized. A characteristic three-dimensional Immunoband will be formed if the test is positive. Results are read visually |
of enrichment to the VIP device and read results in just 10 minutes. 
